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8\m=.\3 \g=m\gDHT/day escaped from the capsule over the course of the experiment compared to 12\m=.\0 \g=m\gtestosterone/day. In order to compare the maintenance of spermatogenesis in DHT- and testosterone-treated rats with that in normal controls, experiments were undertaken in which larger quantities of hormones than those in the previous study were administered to hypophysectomized rats. Twenty-eight male Long\p=m-\Evansrats were hypophysectomized at 90 days of age by the parapharyngeal approach and were randomly assigned to one of three treatment groups. Treatment was initiated immediately after hypophysectomy and consisted of daily subcutaneous injections of 1 mg DHT (Group 1) or 1 mg testosterone (Group 2) dissolved in 0\m=.\1 ml corn oil. Hypophysectomized controls were given 0\m=.\1 ml corn oil daily (Group 3). Six untreated intact rats (Group 4) completed the experimental design. The animals were housed at 35\s=deg\C in a light\x=req-\ controlled room (14 hr dark/10 hr light) and were allowed free access to Purina Lab Chow and water. In order to ascertain any effects on mating behaviour as well as the capacity of spermatozoa from animals in Groups 1 and 2 to fertilize, a nulliparous Sprague-Dawley female was placed with each male for the last week of the study (Days 22 to 28). The females were kept under observation to term and the number of young born was recorded. After 4 weeks of treatment, the males were killed ; ventral prostates, seminal vesicles and testes were dissected free, weighed and placed in Bouin's solution. The head of the epididymis was cut and a smear was made on a slide and examined under a microscope for the presence of motile spermatozoa. All of the * Deceased.

MAINTENANCE OF SPERMATOGENESIS WITH TESTOSTERONE OR DIHYDROTESTOSTERONE IN HYPOPHYSECTOMIZED RATS