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The proliferation of trophoblast from the mouse blastocyst in vitro has been analysed by Gwatkin (1966a, b) and Gwatkin & Meckley (1965, 1966). This outgrowth occurs when blastocysts are cultured in Brinster's medium (Brinster, 1965) supplemented with fetal calfserum. The present work was undertaken to determine if this outgrowth would also occur when either normal mouse serum or serum from mice with ascites teratomata was used. This tumour was chosen since its ascites form is composed of embryoid bodies, groups of cells morpho¬ logically similar to early mouse embryos (Stevens, 1960). All sera were collected by killing mice with chloroform and withdrawing blood by heart puncture. The blood was allowed to clot at 4°C for 12 hr, after which it was centrifuged, and the serum was decanted. All sera were stored at

INHIBITION OF BLASTOCYST OUTGROWTH IN VITRO BY SERUM FROM MICE WITH ASCITES TERATOMA