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Work on pregnant mares serum gonadotropin PSMG treated rats and induction of ovulation with progesterone is reviewed. The progesterone pregnenolone and related metabolites were examined for the facilitative action in 22 day old rats treated with a nonovulatory dose of PMSG. Progesterone (.5 mg) induced ovulation in all rats treated with 12 iu of PMSG. 1.5 mg of pregnenolone given at 10 hours on Day 24 produced ovulation in 80% of the animals. Neither 17 alpha-hydroxy pregnenolone nor 17 alpha-hydroxyprogesterone had any facilitative effect. 1.5 and 2 mg of 5 alpha-dihydrogesterone caused ovulation in all treated animals. 3 alpha-hydroxy-5 alpha-pregnan 20-one had no effect. The action of pregn-5-ene-3 20-dione was equal to that of progesterone in doses of .25 mg or higher.

EFFECT OF 5 -DIHYDROPROGESTERONE, PREGN-5-ENE-3,20-DIONE, PREGNENOLONE AND RELATED PROGESTINS ON OVULATION IN PMSG-TREATED IMMATURE RATS