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Levels of lh were determined by radioimmunoassay and those of progesterone by a competitive protein-binding technique. Blood samples from eight cows, representing two complete pregnancies (including post-partumperiods), and from one cow in the first trimester and another around the time of parturition, were collected. The samples were withdrawn at intervals of 6 hr for lh and every 4 to 5 days for progesterone determination. Levels of lh were consistently low (1\m=.\0to 1\m=.\6 ng/ml plasma) with a few single peaks occurring irregularly in individuals during the first 110 days. Distinct lh peaks were observed between Days 7 and 20 post partum. The decrease of progesterone from high levels during pregnancy (plateau between 5 and 9 ng/ml plasma with individual differences) before parturition and the low level following was not re-

reproduction

SIMULTANEOUS DETERMINATION OF LH AND PROGESTERONE IN PERIPHERAL BOVINE BLOOD DURING PREGNANCY, NORMAL AND CORTICOID-INDUCED PARTURITION AND THE POST-PARTUM PERIOD