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Chemical analyses were conducted on uterine fluid and blood serum collected from eighty-eight normal adult dairy cows during various days of the oestrous cycle. Uterine fluid contained higher concentrations of reducing substances, potassium, inorganic phosphate and alkaline and acid phosphatase activity than blood serum while levels of calcium and sodium were higher in blood serum than in uterine fluid, supporting the concept that bovine uterine fluid is a product of active secretion and not of simple diffusion from the blood. Concentrations of reducing substances, total protein, potassium, chloride, inorganic phosphate and alkaline and acid phosphatase activity in bovine uterine fluid were all found to vary with the stage of the oestrous cycle, indicating that, in the case of these substances at least, secretion into the uterine lumen is under hormonal control. The level ofalkaline phosphatase in the blood serum was also found to vary with the stage of the oestrous cycle.

A CHEMICAL STUDY OF UTERINE FLUID AND BLOOD SERUM OF NORMAL COWS DURING THE OESTROUS CYCLE