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Summary. When ram semen was dialysed against phosphate-glucose diluents with a cation composition similar to rete testis fluid, epididymal and ejaculated seminal plasma and ejaculated ram spermatozoa, the impedance change frequency (ICF) was maintained best over 6 hr in the latter diluent. Krebs-Ringer-glucose buffered with sodium phosphate or trisodium citrate maintained the ICF at a high level, whereas tris-HCl and veronal-HCl buffers caused a rapid decline in ICF. Hypertonic dia- lysis media severely depressed the ICF within 1 hr, whereas hypotonic diluents gave results similar to Krebs-Ringer-phosphate. Adding 1 mm-calcium or 5 to 51 mm-potassium to an isotonic dialysis fluid was beneficial and the effects on ICF were additive. The omission of potassium from the hypotonic diluent caused no significant reduction in ICF.

USE OF A CONTINUOUS FLOW DIALYSIS APPARATUS TO DETERMINE THE EFFECT OF CATIONS, BUFFERS AND OSMOTIC PRESSURE ON RAM SPERMATOZOA