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Summary. In nine out of eleven operations, fistulae were established in the vas deferens of rams. This was accomplished by incising the vas deferens and suturing the orifice to a small surgical opening in the dorsal surface of the scrotum, close to the incision through which the vas deferens had been exposed. In order to obtain epididymal semen from live animals, fistulae have been established in the vas deferens of bulls and rams by a small number of investi¬ gators. Bennett & Rowson (1963) cannulated the vas deferens of one bull from the posterior side of the scrotum. Amann, Hokanson & Almquist (1963) used a cannula and a special collection system attached to the scrotum for obtaining epididymal semen from two bulls. White, Larsen & Wales (1959) made fistulae in the vas deferens of rams on the anterior surface of the scrotum by securing the wall of the incised vas to the margin of the surgical opening of the skin. Some of their fistulae remained patent for considerable lengths of time. In short-term experiments, Ewy, Bielanski & Zapletal (1963) obtained epididymal semen from rams through a cannula inserted into the vas deferens from the anterior side of the scrotum. In our previous experiments, we obtained fistulae of the vas deferens of rams by using a polyethylene cannula which was inserted into the vas deferens and held in place by a silver disc sewn to the scrotal skin (Tadmor & Schindler, 1966).

A FURTHER APPROACH TO THE ESTABLISHMENT OF A FISTULA IN THE VAS DEFERENS OF RAMS