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on the quality of buffalo semen compared with other cattle (Sinha, Gupta & Roy, 1966). Semen was collected from four Murrah buffalo and four Hariana bulls and the study was made during the seasons mentioned in Table 1. Immediately after collection, semen was stored in a thermos flask containing ice. The spermatozoa and seminal plasma were separated by centrifuging a known volume ofpooled semen at low temperature for halfan hour. The chemical constituents were determined according to the standard methods described by Hawk, Oser & Summerson (1954). The sodium content of the seminal plasma of the two species did not differ significantly, but seasonal differences were noted, indicating higher concentra¬ tions in cold, and slightly lower values in hot and hot-humid climates. The potassium and chloride contents of the seminal plasma and the potassium content of the buffalo spermatozoa were higher (P<0\m=.\01) than those of the bulls in all seasons. Steinbach & Dunham (1961) demonstrated that the motility of the sperma¬ tozoa of Arbacia punctulata depended upon the ion gradient (sodium and potas¬ sium) in the sperm cells. Cragle & Salisbury (1959) showed that potassium levels in diluting media, comparable to those found in seminal plasma, had an inhibitory effect on the oxygen consumption, fructose utilization and lactic acid accumulation of bull spermatozoa. These reports indicate that the higher potassium concentrations found in the seminal plasma as well as the sperma¬ tozoa of buffalo might, to some extent, be associated with the lower survival of these spermatozoa under storage conditions. The inorganic phosphorus and calcium contents of the seminal plasma of the two species did not differ significantly, nor did they differ significantly from

CHEMICAL COMPOSITION OF CATTLE AND BUFFALO SPERMATOZOA AND SEMINAL PLASMA UNDER DIFFERENT CLIMATIC CONDITIONS