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Comparative studies of semen characteristics in subhuman primate species should be of value in the selection of appropriate species for experiments in reproduction. In addition, basic information obtained in such studies may be helpful for the successful preservation of frozen semen and insemination. These techniques will probably be essential in the maintenance of large primate colonies. Finally, comparative information of this kind should be of interest to students of primate classification. Animals providing specimens for this study were selected from over 800, representing a dozen or more species, located at the Delta Regional Primate Research Center, Covington, Louisiana. The classification employed is that outlined in the Progress Report of the Primate Centers' Committee on Primate Nomenclature (G. G. Simpson, Chairman). Semen specimens were collected from anaesthetized animals by electro-ejaculation (Roussel & Austin, 1968). They were incubated at room temperature for 30 min with an equal volume of 1% trypsin, in order to digest the coagulum; the coagulum appears in most ejaculates, and contains a concentration of spermatozoa about equal, by volume, to that of the liquefied plasma (Roussel & Austin, 1967a, b). Volume, sperm concentration, and the percentage of motile, eosin-negative, and morphologically abnormal cells were recorded. The specimens were sealed in 1 \m=.\2-mlampoules, and frozen in liquid nitrogen. All specimens were collected within a 60-day period beginning 14th September 1966. Human semen specimens were collected in Ann Arbor, and were treated and preserved in the same way. For subsequent studies (in Ann Arbor), the thawed cell suspensions were centrifuged at 800 g ; the seminal plasma was taken off, and the packed cells

FRUCTOSE, LACTIC ACID AND CITRIC ACID CONTENT OF THE SEMEN OF ELEVEN SUBHUMAN PRIMATE SPECIES AND OF MAN