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Ovarian slices from non-pregnant and 6-day-pregnant rabbits were incubated with [1-14C]acetate. 14CO2 was collected, and subsequently the amounts, specific radio-activities, fatty acid composition and fatty acid specific activities of the isolated phospholipids were determined. Phosphatidyl choline was increased in amount in the ovary during pregnancy, and [1-14C]acetate incorporation into phosphatidyl choline and phosphatidyl inositol was also increased. 14CO2 production was greater from ovaries of pregnant than non-pregnant rabbits. The percentage of oleic acid in both phosphatidyl choline and phosphatidyl ethanolamine was higher in ovaries from the pregnant rabbits, and palmitic acid in phosphatidyl ethanolamine was slightly decreased during pregnancy. Of the major fatty acids of phosphatidyl choline, only palmitic acid showed an increased radio-acetate incorporation with pregnancy.

reproduction

OVARIAN PHOSPHOLIPID COMPOSITION AND INCORPORATION OF [1-14C]ACETATE INTO THE PHOSPHOLIPID FATTY ACIDS OF OVARIES FROM NON-PREGNANT AND PREGNANT RABBITS