English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The secretion of citric acid by the bovine female genital epithelium has been investigated by Gaspar & Cseh (1963). In 60% of cases no citric acid was present, but in the secreta of 40 % of the cows, quantities corresponding to spots of 1 \m=.\5to 40 \g=m\gcould be demonstrated. In 30 % of the investigated cases one unidentified spot, usually accompanied by citric acid and showing Rf values from 0\m=.\78to 0\m=.\80,also appeared on the chromatogram. We have been trying to identify more closely the compound forming this spot. It was diffusible and remained unhydrolysed in 1 n-sulphuric acid for 2 hr and was in character acid. Although the unknown compound was generally accompanied by citric acid, it could not be identified either with lactic acid, pyruvic acid and the components of the citrate cycle, or with maleinic and citraconic acid. Oestrous secreta from eighty-four living dairy cows and sixty slaughtered animals were used as test material. The material from living cows was collected directly from the os of the cervix by aspiration with a syringe provided with a catheter. Uterine mucus, oviduct fluid, and follicular fluid was collected from slaughtered animals directly from the organ. The method of paper chromatography described by Schreier & Hack (1956) was used. The absorption spectrum of the stained compound, eluted with ethanol, was measured with a Beckman spectrophotometer. The unknown compound is supposed to be closely related to or even identical with hippuric acid, which is found in exceedingly large quantities in herbivora, as a final product of the detoxication of benzoic acid and its derivates. This conclusion is based on the following considerations. (a) The comparison made between the chromatographic data of Gaffney, Schreier, DiFerrante & Altman (1954) and our own results (see Table 1). (b) The absorption maximum of the substance tested by Gaffney et al. (1954) was at 460 m\g=m\,while that of the substance deriving from the secreta and the absorption maximum of hippuric acid under similar conditions were found at 475 m\g=m\(see Gaffney et al. Fig. 1 and Text-figs. 1 and 2). (c) In-vivo load tests also prove that the investigated substance is a benzoic acid derivative. Sixty to 90 mg/kg of sodium benzoate was administered intravenously to five cows. The substance tentatively identified as

BENZOIC ACID DERIVATIVE IN THE SECRETA OF THE GENITAL EPITHELIUM OF THE COW