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All 73 sera were analyzed for t-25OHD by two assays with different principles. In addition the following biomolecules were measured: PTHbiointact (Bio-PTHi) by immunometric assay (ECLMA, Roche), vitamin D binding protein (DBP) by turbidimetry (Dako), and total protein (TP), albumin (Alb) and calcium (Ca) by colorimetry (Roche, Modular). The bio-25OHD values were calculated (Vermeulen et al. 1999, Bhan et. al. 2012) The cutoff values for evaluation of Vitamin D supply or t-25OHD and bio-25OHD are summarized in the table.

Evaluation of two routinely used 25OHD assays and serum variables in patients on dialyses