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Conclusions These results show that variations in FBS have a significant effect on spontaneous mineralization rates in acellular SF scaffolds. We need to learn more about the process of mineralization and how variations in media composition affect tissue calcification in 3D cell culture. Results & Discussion Though we expected calcium content to remain low in acellular scaffolds, by week 7 the calcium content in some acellular scaffolds was nearly half the amount of their cellularized counterparts. Calcium content varied significantly between FBS brands in acellular scaffolds, but not in cellularized scaffolds, and μCT analysis showed similar trends. PAA Gold and PAA Standard Quality both displayed relatively low levels of mineralization when compared to Biochrom’s FBS Superior or FBS Standard from Gibco.

The choice of fetal bovine serum influences the degree of spontaneous mineralization on silk fibroin scaffolds in 3D cell cultures