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In our study of sample stability over time, samples dehydrated in ethanol showed a loss of reliable signal after 20min in ambient conditions, while populations dehydrated in methanol did not show a decreased signal after 120min (figure 2). Therefore, methanol dehydration was used in all following studies. Next, gene expressions of specific tissue samples were analyzed. Expressions of SOST, DMP1, and ALPL were analyzed relative to the expression of a housekeeping gene, HPRT1 (figure 3). As expected, osteocytes expressed significantly higher levels of both osteocyte markers, SOST and DMP1 (p<0.05). Results

Single osteocyte gene expression in an in vivo model for load-induced bone adaptation