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A first observation of predation on an arachnid by a crustacean, the crab Aratus pisonii (H. Milne-Edwards, 1837) (Brachyura: Sesarmidae) is recorded here. In this case on the ghost spider Hibana talmina Brescovit, 1993 (Araneae: Anyphaenidae: Anyphaeninae), in the Caribbean region of Colombia, at Caño del Oro, Tierra Bomba island (Cartagena), Bolívar. How to cite this article: Galvis W., Gabb A., Vergara-Moreno D. 2019. First observation of arachnid predation (Araneae: Anyphaenidae: Hibana talmina) by a crustacean (Brachyura: Sesarmidae: Aratus pisonii) // Invert. Zool. Vol.16. No.4. P.405–408. doi: 10.15298/ invertzool.16.4.08