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Parasitic Root-Lession nematode of Pratylenchus coffeae can reduce the Indonesian coffee plants productivity. Several studies reported that Pseudomonas fluorescens and Bacillus subtilis endophytic bacteria were antagonistic bacteria to nematode. The objective of this research was to reveal the effectiveness of bacterial colonies density of P. fluorescens (UB_Pf1), B.subtilis (UB BS1), and a combination of both bacteria on nematode mortality using median lethal concentration (LC 50 ) and median lethal time 50 (LT 50 ). The densities of bacteria used in this study were 10, 10, 10 and 10 cfu/ml. 35 testing nematodes were used and the mortality was counted at 6, 12, 24, 36, and 48 hours after treatments. The results showed that LC 50 values of P. fluorescens was (UB_Pf1) was 4,3x10 cfu/ml, LC 50 B. subtilis (UB_Bs1) was 1,9x10cfu/ ml, and LC 50 combination of both bacteria was, 8x10 cfu/ml. It implies that the application of the combination of both bacteria are more pathogenic than single bacterial treatment. The results also showed that the highest LT 50 value was 13.21 hours combination of bacterial colonies with a density of 10 cfu/ml and the lowest LT 50 value was 52.00 hours on P. fluorescens (UB_Pf1) treatment with colonies density of 10 cfu/ml.

biosaintifika

The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae)