English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Medinilla formosana Hayata, a beautiful endangered shrub of the family Melastomataceae endemic in Taiwan of China, is recalcitrant for micropropagation in vitro until now. And tissue browning after cutting generally occurred in the tissue culture of Medinilla species was considered to be the greatest obstacle. To avoid the serious cut-browning, immature ovaries in 0.3 cm diameter of M. formosana were taken as initial explants in this study, and its aseptic plantlets were obtained for the first time. The highest survival level (12.7 plantlets per ovary) after 3 months of culture were observed on 1/2 MS supplemented with 0.5 g l-1 active carbon and 0.4 g l-1 casein hydrolysate (pH 5.5). Furthermore, β-mercaptoethanol proved to be an effective inhibitor for its browning response of M. formosana in vitro. And 1/2 MS media supplemented with 6.67 μM BA, 0.11 μM NAA, 0.5 mM β-Met and 0.2 g l-1 AC proved appropriate for its multiplication. Finally rooted plantlets were transplanted into the greenhouse after acclimatization, and the micropropagation system of M. formosana was successfully established. This report first details an efficient method for the in vitro micropropagation of Medinilla plants.

An effective route for the micropropagation of Medinilla formosana through ovary culture in vitro