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It is well known that living organisms can be undercooled to certain low temperatures. Most of them are not injured by undercooling itself, but when freezing once occurs within their tissues they suffer from frost-injury. The degree of such an injury is various according to the kinds of organisms. The great majority of works on the relation between capacity for undercooling and cold resistance have dealt with animal materials, especially with insects. Salt' showed that a high capacity of undercooling is regarded as the essential factor of cold resistance in the non-frost-resistant insects. According to Aoki3~ , the value of undercooling point** can not be an index of f ros c resistance because it does not differ in insects with different frost resistance. In a potato tuber it was shown by Terumoto3~ that undercooling point was lowered during the hardening process. However, whether the undercooling is an important factor in frost resistance or not is still uncertain4~ . The purpose of the present investigation is to clarify the relation between degree of undercooling and frost resistance in leaf tissue. Since the undercooling point is, as a rule, affected by several physical factors, especially by the rate of cooling, the effect of cooling rate on the undercooling points in leaf tissues is also reported here.

Undercooling Points and Frost Resistance in Mature and Immature Leaf Tissues of Some Evergreen Plants