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Growth characteristics of medicinal mushroom Grifola frondosa mycelia were studied in solid-state cultivation on various solid-state substrates in different setups. The mycelial growth rate was determined in racing tubes by the measurement of ergosterol content. The fastest growth rate of 3.76 mm d –1 and the highest biomass amount of 54 mg g –1 were achieved. The results were scaled-up in a horizontal stirred bioreactor, where higher amounts of biomass (53.2 mg g –1 in 42 days) were achieved. These results represent an available platform for large-scale production of medicinal fungi biomass in bioreactors.

Growth Characteristics and Ergosterol Content of Grifola frondosa in Various Solid-state Substrates