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The present study used the method of embedding-adsorption to immobilize fungal laccase and to determine the suitable conditions of immobilization by measuring various activities of immobilized laccase. In addition, the immobilized laccase was further employed to repair the carbofuran-contaminated soil, and then the degradation rates of carbofuran were measured under different conditions. The experimental results showed that the appropriate conditions of embedding-adsorption were presented as follows: the weight of powdered active carbon (PAC) was 0.4 g, the concentration of CaCl2 was 1.5%, the volume of crude laccase was 80 ml, and the immobilized time was 6 h. After 48 h, the degradation rate of carbofuran in soil could reach almost 86% by the immobilized laccase. In fact, the artificially polluted soil and polluted soils in the environment have many differences, so the coming experiment will be concentrated directly on contaminated soil based on laboratory studies in order to investigate the influence of various factors on in-situ remediation.

Degradation of Carbofuran in Contaminated Soil by Immobilized Laccase