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This study was conducted to identify the morphology of digestive tract of stick insect, Phobaeticus serratipes (Phasmida: Phasmatidae) as well as its histological structure. The distribution of biogenic amine, serotonin on the structure was also determined. Samplings for this study were conducted in highland areas in Langkawi Island, Kedah, Fraser Hill, Pahang and Mount of Ledang, Johor. The in-situ and ex-situ observation on gross morphology of digestive tract was done using stereo microscope Stemi v10 that was connected with DSLR Canon EOS 6D camera. The histological study of the alimentary tract involves special staining procedures of Periodic Acid Schiff‘s reagent and Alcian blue method. Serotonin distribution was conducted using immunohistochemical method with Dylight as secondary antibody. The serotonin distribution was observed under fluorescence microscope Zeiss Axiocam MRm Apotome.2 with ZenPro 2012 software and also Olympus FSX100 microscope. Digestive tract morphology and histology showed that P. serratipes foregut is the longest section consists of the oral cavity, pharynx, esophagus, crop and proventriculus. Gastric caeca and ventriculus embodied the midgut area. Midgut having columnar epithelial cells as the major cells that serve its purposes in nutrient absorptions and enzymatic metabolisms. The hindgut was the shortest part of the digestive tract that consists of Malpighian tubules, ileum, colon, rectum and anus. The serotonin of digestive tract were scattered and disorganized and mostly found on the muscle cells. Keywords–-Histology, stick insects, Phobaeticus serratipes, digestive tract, serotonin

Morphology, Histology And Serotonin Distribution On Digestive Tract Of Stick Insect, Phobaeticus serratipes (Phasmida: Phasmatidae)