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The fluorescence quenching of Eosin by certain dyes has been investigated using steady state technique. The dyes used are Uniblue-A, Acid blue 129, Alizarin, Alizarin red S, Erichrome Black T, Tartrazine, Methyl orange, Methyl red, Acid orange 63, Congo red. The quenching was found to obey the Stern-Volmer equation and the corresponding Stern-Volmer plots were linear in the range of quencher concentration used [0−5×10 −  5 ] M. The quenching rate constant ( k   q  ) is in the range of 0.85–26.65×10 12  M −  1 s −  1 . The quenching mechanism is discussed on the basis of the quenching rate constants as well as the reduction potential of the dyes. Rehm-Weller equation is applied for the calculation of free energy change (ΔG et ).

A Study on the Fluorescence Quenching of Eosin by certain Organic Dyes