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The conditional stability constants for trace-level concentrations of Pu(IV) complexing with galacturonic acid and EPS, isolated from axenic   Clostridium   sp.,   P. fluorescens   and   Shewanella putrefaciens   CN32 cultures, were determined at pH 4 and an ionic strength of 0.1 M NaCl using an ion-exchange technique. The analysis of ion-exchange data with Schubert´s technique indicates that the Pu binding by galacturonic acid and EPS from   Clostridium   sp. and   S. putrefaciens   can be described based on the formation of 1:1 Pu(IV)-ligand complexes. However, the accurate description of Pu binding by EPS from   P. fluorescens   requires postulation of a mixture of 1:1/1:2 complexes between Pu(IV) and ligands under the experimental conditions studied.

radiochimica acta

Binding of Pu(IV) to galacturonic acid and extracellular polymeric substances (EPS) from  Shewanella putrefaciens, Clostridium  sp. and  Pseudomonas fluorescens