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The purpose of this study was to analyse the volatile components of Thymus magnus Nakai extracted by different extraction methods and reproduce scent close to original plant based on the results. For this purpose, the essential oil of T. magnus was extracted by supercritical fluid extraction (SFE), water and steam distillation (WSD) and simultaneous steam distillation and extraction (SDE) methods. The compositions of the essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS). Consequently, linalool (0.1%) and trans-sabinene hydrate (0.9%) contents in the essential oil extracted by SFE method of - 400 bar condition were relatively higher than compositions of the essential oil extracted by different conditions. The contents of borneol (3.82%), terpinen-4-ol (0.3%) and caryophyllene oxide (2.2%) were relatively higher at - 400 bar and the contents of -bisabolene (5.88%), 1-octen-3-ol (0.31%), caryophyllene (2.91%), p-cymene (2.04%) and -terpinene (0.52%) were extracted relatively higher at - 300 bar. The compositions of the essential oil extracted by SFE method of - 200 bar condition contained relatively higher contents of thymol (77.63%) and carvacrol (5.65%). The contents of -bisabolol (0.17%), caryophyllene (6.46%), cis--bisabolene (1.52%) and -bisabolene (20.65%) in the essential oil extracted by WSD method were relatively higher than compositions of the essential oil extracted by SFE method, and by SDE method we couldn`t obtained essential oil. The results of this study could be utilized to reproduce scent close to original scent of T. magnus.

Comparison of Volatile Compounds from Thymus Magnus Nakai by Three Different Extraction Methods