English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The functional expression of large-conductance Ca2+-activated K+(KCa) channels in lumbar motoneurons (LMNs) of the developing chick embryo is regulated in part by interactions with striated muscle target tissues. Here we show that the functional expression of KCa channels in LMNs developingin vitro can be stimulated by application of a skeletal muscle extract (MEX) or by coculture with hindlimb myotubes. A similar stimulation of KCa channels in vitro can be produced by the trophic factors glial cell line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor but not by neurotrophin (NT)-3 or NT-4. The actions of MEX and hindlimb myotubes are blocked by a GDNF-neutralizing antiserum. Moreover, injection of this same antiserum into the embryonic hindlimb reduced the functional expression of KCa channels in vivo to levels seen in LMNs deprived of interactions with the hindlimb. The effects of GDNF on KCa channel expression in LMNs require 24 hr of continuous exposure to reach maximum and are blocked by the translation inhibitor anisomycin, indicating the need for synthesis of new proteins. GDNF actions are also blocked by the farnesyl transferase inhibitor manumycin, suggesting a role for Ras in the actions of GDNF. Finally, the actions of GDNF are inhibited by PP2, an inhibitor of Src family tyrosine kinases, and by LY29003, an inhibitor of phosphatidylinositol 3 kinases, but not by PD98059, an inhibitor of the Erk signaling cascade. None of these treatments alter expression of voltage-activated Ca2+ channels. Thus, the actions of GDNF on LMN KCa channel expression appear to use a transduction pathway similar to that used for regulation of apoptosis.

the journal of neuroscience/the journal of neuroscience

Glial Cell Line-Derived Neurotrophic Factor and Target-Dependent Regulation of Large-Conductance K<sub>Ca</sub>Channels in Developing Chick Lumbar Motoneurons

Glial Cell Line-Derived Neurotrophic Factor and Target-Dependent Regulation of Large-Conductance KCaChannels in Developing Chick Lumbar Motoneurons

Glial Cell Line-Derived Neurotrophic Factor and Target-Dependent Regulation of Large-Conductance K_CaChannels in Developing Chick Lumbar Motoneurons