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every year [3]. Due to adverse environmental effects associated with chemical insecticides have led to the search for an alternative methods for controlling different disease-transmitting mosquito species [4]. Over the past 20 years, botanical insecticides have thrown an attention as new approaches to insect control [5]. In India has rich in aromatic plant diversity with potential for development of natural insecticides for control of mosquito and other pests [6]. In this regards plant seed contain several biologically active proteins that play a various specialized functions, in these the most representative molecules are hydrolytic enzymes, inhibitors, lectins and the ribosome inactivating proteins [7, 8]. Among these several insecticidal molecules, the lectin play a major role in control of several insect pest order such as Coleoptera, Diptera, Lepidoptera and Homoptera, due to these protein can act as recognition molecules in cell–cell or cell–matrix interactions [9] and may bind to the peritrophic membrane and especially it has ability to bind to carbhohydrate chain moieties on insect gut membrane and it has some characteristic features that these protein are resistant to proteolytic degradation by insect digestive enzymes, therefore it has inhibit the food digestion, absorption and consequentially the larvae was dead. [9-12]. Lectin from Myracrodroun heartwood, bark and leaf showed effective larvicidal activity of Aedes aegypti [13]. In addition, Coelho et al [14] reported that WSMoL showed larvicidal activity in the lethal concentration values of (LC50 0.197 mg/mL) against the (L4) of Aedes aegypti. The Manilkara zapota belongs to the family of sapotacea, Genus: Manilkara, Species: zapota and it has become a major commercial crop in India. It is growing to a height of around 8 meters. The bark is dark brown and deeply fissured and fruit is brown, fleshy, ovoid to round 3-8 cms long and fruit containing 5 or more shiny blackish brown seeds [15-17]. The plant material has various biological activities such as the leaves have larvicidal activity of Haemaphysalis bispinosa, Hippobosca maculata, and Anopheles subpictus [18] anti-microbial, antibacterial [19, 20] and antioxidant properties [21]. Seed protein are small hydrophilic proteins, ranging from 83 to 153 amino acid residues, performing a number of crucial physiological function mainly seed has sapling defense [22]. Till date there is no report available using crude protein extract to Abstract

Entomotoxicity Properties of Eco-Friendly Crude Protein Extract From Manilkara Zapota Seed against Asian Tiger Vector Aedes Aegypti