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A QuEChERS-based extraction protocol combined with a fast and selective UHPLC-QTOF-MS assay for the detection and quantification of destruxins from honey is described. The internal standard fortified assay showed satisfying assay precision and accuracy and allowed quantification of destruxin A and B down to ppb range with limits of detection (LOD) and quantification (LOQ) of less than 1.0 ppb and 2.3 ppb, respectively. Destruxin E showed a distinctive loss of recovery and was excluded from further quantitative analysis. Honey samples obtained from a field trial using Metarhizium brunneum against the ectoparasitic honey bee mite Varroa destructor were examined for possible destruxin residues. No analytes could be detected and samples were found to be residue-free.

soj pharmacy and pharmaceutical sciences

Combination of A Quechers-Based Extraction Protocol with A Fast and Selective UHPLC-QTOF-Ms assay For The Detection And Quantification Of Metarhizium Brunneum Metabolites From Honey samples