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The internode segments of Giao Co Lam (Gynostemma pentaphyllum) sterilized with diluted solution of javel (50%) for 20 minutes reached to 73.33% sterile explants. In vitro cutting stem segments of Giao Co Lam cultured on MS medium supplemented with 1.0 mg/L BA and 0.1 mg/L NAA gave the highest shoot induction (6.8 shoots/ explant). The regenerated shoots cultured on MS 1⁄2 medium were suitable for growth of shoots with 5.2 cm in height and 4.0 leaves per plantlet. For root induction, the MS 1⁄2 medium supplemented with 0.25 mg/L IBA was appropriate and the root length could be in 7.6 cm in this medium. The rate of survival plantlets is 85% in garden. Keywords—Gynostemma pentaphyllum, multiplication of Giao Co Lam, medicinal plant, shoot proliferation

Preservation and development of Giao Co Lam Gynostemma pentaphyllum in the hot tropics of Vietnam