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A high percentage of agricultural regions are affected by salinity and drought (1). Therefore, these osmotic stresses are considered as the most important environmental factors affecting plant productions. Members of a family, genus or even species use different mechanisms in response to salt stress (2). In Agriculture, different responses to salt stress in close relatives of crops are of interest in order to determine salt tolerant mechanisms. Biological responses and developmental programming of cells are eventually controlled by precise regulations of gene expressions (2). While comparative genetic mapping in plants has provided evidence for a remarkable conservation of marker and gene order (colinearity) between related grass genomes (3), still reliable methods are required to reveal the gene expression profiles in various environmental conditions and/or developmental stages. Many molecular techniques are now available to identify and clone differentially regulated genes. In 1992, a PCR-based method, collectively referred to as differential display (DD) or RNA fingerprinting was developed in which a set of arbitrary primers were used to amplify cDNA fragments representing Iran J Biotech. 2014 December;12(4): e1121 DOI:10.15171/ijb.1121

iranian journal of biotechnology

The Application of a Non-Radioactive DD-AFLP Method for Profiling of Aeluropus lagopoides Differentially Expressed Transcripts under Salinity or Drought Conditions