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Advanced biotechnology based on genetic engi-neering techniques open a window of opportunity intransgenic animals and livestock improvement. One ofthe main aspects in the creation of transgenic animalsis gene targeting technology, which allows insertingthe exogenous genes in the identified sites in mam-malian genome. Phic31 integrase system has beendeveloped for site specific gene integration and used asa tool for gene targeting in mammalian genome modi-fication (1). This system mediated by a member of ser-ine-catalyzed enzymes encoded by phiC31 phage. Thisphase initiates its life cycle by integrating its wholegenome into specific sites named

Identification of a Specific Pseudo attP Site for Phage PhiC31 Integrase in Bovine Genome