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Purpose:  Implication of protein-protein interactions (PPIs) in development of many diseases such as cancer makes them attractive for therapeutic intervention and rational drug design. RON (Recepteur d'Origine Nantais) tyrosine kinase receptor has gained considerable attention as promising target in cancer therapy. The activation of RON via its ligand, macrophage stimulation protein (MSP) is the most common mechanism of activation for this receptor. The aim of the current study was to perform in silico alanine scanning mutagenesis and to calculate binding energy for prediction of hot spots in protein-protein interface between RON and MSPβ chain (MSPβ).  Methods:  In this work the residues at the interface of RON-MSPβ complex were mutated to alanine and then molecular dynamics simulation was used to calculate binding free energy.  Results:  The results revealed that Gln 193 , Arg 220 , Glu 287 , Pro 288 , Glu 289 , and His 424 residues from RON and Arg 521 , His 528 , Ser 565 , Glu 658 , and Arg 683 from MSPβ may play important roles in protein-protein interaction between RON and MSP.  Conclusion:  Identification of these RON hot spots is important in designing anti-RON drugs when the aim is to disrupt RON-MSP interaction. In the same way, the acquired information regarding the critical amino acids of MSPβ can be used in the process of rational drug design for developing MSP antagonizing agents, the development of novel MSP mimicking peptides where inhibition of RON activation is required, and the design of experimental site directed mutagenesis studies.

Characterizing the hotspots involved in RON-MSPβ complex formation using in silico alanine scanning mutagenesis and molecular dynamics simulation