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The aim of this research was to evaluate insecticidal activities of the essential oil of  Rhynchanthus beesianus  rhizomes against adults of  Liposcelis entomophila  and  Tribolium castaneum . Gas chromatography-mass spectrometry analyses revealed the presence of 44 compounds with  β -eudesmol (19.1%), elemol (8.1%),  α -terpineol (8.0%), methyl eugenol (6.5%), and caryophyllene (4.8%) being the major constituents. Bioactivity-directed chromatographic separation of the oil led to the isolation of four constituents, elemol,  β -eudesmol, methyl eugenol, and  α -terpineol. The essential oil exhibited fumigant toxicity against the adults of  L. entomophila  and  T. castaneum  with LC 50  values of 0.57 and 4.96 mg/L air while the two isolates, methyl eugenol and  α -terpineol possessed fumigant toxicity against the booklice (LC 50  = 0.15 and 0.48 mg/L air, respectively) and the beetles (LC 50  = 1.81 and 4.96 mg/L air, respectively). The oil also possessed contact toxicity against the booklice and the beetles with LD 50  values of 121.56 μg/cm 2  and 54.93 μg/adult, respectively, while the two isolates  β -eudesmol and elemol showed contact toxicity against  L. entomophila  (LD 50  = 99.21 and 35.19 μg/cm 2 , respectively) and  T. castaneum  (LD 50  = 35.26 and 8.89 μg/adult, respectively). The results indicate that the oil of  R. beesianus  rhizomes and its isolates have potential as a source for natural insecticides.

Insecticidal activities of the essential oil of Rhynchanthus beesianus rhizomes and its constituents against two species of grain storage insects