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Bioactivity-guided phytochemical investigation of  Cucumis bisexualis  has led to the isolation of three new coumarin-aurone heterodimers ( 1 - 3 ), along with six aurone derivatives ( 4 - 9 ) were isolated from  C. bisexualis  for the first time. Their structures were determined by their extensive spectroscopic data and comparison with the values reported in the references. All isolated compounds ( 1 - 9 ) were evaluated for their hepatoprotective activities on human L-O2 cells, which compared with positive control of Bifendatatum. Among them, compounds  1 ,  2 , and  7  exhibited moderate hepatoprotective activities to promote effects on the proliferation of L-O2 cells.

Bioactivity-guided isolation of aurone derivatives with hepatoprotective activities from the fruits of Cucumis bisexualis