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The endophytic fungus  Mycosphaerella  sp. (UFMGCB2032) was isolated from the healthy leaves of  Eugenia bimarginata , a plant from the Brazilian savanna. Two novel usnic acid derivatives, mycousfuranine ( 1 ) and mycousnicdiol ( 2 ), were isolated from the ethyl acetate extract, and their structure was elucidated by NMR and MS analyses. Compounds  1  and  2  exhibited moderate antifungal activities against  Cryptococcus neoformans  and  Cryptococcus gattii , each with minimum inhibitory concentration values of 50.0 μg/mL and 250.0 μg/mL, respectively.

Two new usnic acid derivatives from the endophytic fungus Mycosphaerella sp.