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The influence of electric field treatment on dedifferentiation and calli formation on rape hypocotyls was investigated. Segments, 10 mm long, of the upper part of rape (Brassica napus L., cv. GórczaΔski) hypocotyls were stimulated by different combinations of voltage/time (1.5 V/120 h, 3 V/3 h, 10 V/15 min and 30 V/30 s) under in vitro conditions. With all electric field treatments, segments oriented with their apical part towards the cathode produced more calli as compared to control (non-treated with electric field). Under opposite orientation slight inhibition of callus growth was observed. As the strongest effect on callus growth was observed after treatment with 30 V/30 s, this electric field treatment was selected for following analyses: the incorporation of [ 14 C]-2,4-D (2,4-dichlorophenoxyacetic acid) and [ 14 C]- BAP (benzylaminopurine) from the culture medium, changes in ACC (1-aminocyclopropane- 1-carboxylic acid) level and the redox activity in apical and bottom parts of hypocotyls during 18 d of culture.

The Effect of Electric Field on Callus Induction with Rape Hypocotyls