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The effect of extreme temperature on the quantum yield of fluorescence and membrane leakage of Pinus canariensis growing in 5 stands at different altitudes and orientation in Tenerife (Canary Islands, Spain) was determined. Needles were collected from the field and transferred to the laboratory where they were kept in a closed chamber with water-saturated air overnight. Then they were exposed for 30 minutes in plastic bags in a water bath at temperature treatments with steps of 2K between 56 °C and -24 °C and the effect was determined immediately and 24 hours after the treatment by chlorophyll fluorescence and electrolyte leakage.

Effect of Extreme Temperature on Quantum Yield of Fluorescence and Membrane Leakage of the Canarian Endemic Pine (Pinus canariensis)