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A DNA probe specific for biocontrol strains of Pseudomonas was produced by screening randomly amplified polymorphic DNA (RAPD) PCR fragments. Specificity of the probe was assessed by dot blot and colony hybridization. It was used to specifically determine the population of these strains on roots of Cucumis sativus cv. Delikatess. Two polymorphic RAPD fragments of 750 bp, and 550 bp showed identical specificity. The biocontrol strain Pseudomonas sp. W34 was shown to be competitive in the rhizosphere of cucumber and to maintain a stable population for at least 10 days when inoculated on the seed.

zeitschrift für naturforschung c

Biocontrol Strain Pseudomonas sp. W34: Specific Detection and Quantification in the Rhizosphere of Cucumis sativus with a DNA Probe and Genotypic Characterization by DNA Fingerprinting