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We have investigated the influence of Prostaglandin E 1  on the in vitro transcription of chromatin, isolated from spermatozoa of patients suffering from different pathologies, leading to infertility, namely, azoospermia, teratospermia and chronic prostatitis. Our studies indicate that prostaglandin E, has a stimulatory effect both on in vitro transcription, on the number of RNA polymerase molecules and the polyribonucleotide elongation rates as compared to sperm chromatin from healthy patients. Tlie results on the incorporation of α- 32 P-ATP in to RNA in the presence and absence of Prostaglandin E 1  correlate well with the data on the number of actively transcribing RNA polymerase molecules and the rate of RNA elongation, which might be due to low levels of prostaglandin E 1  in human semen.

zeitschrift für naturforschung c

The Effect of Prostaglandin E 1  on in vitro Transcription of Sperm Chromatin, Isolated from Patients with Azoospermia, Teratospermia and Chronic Prostatitis