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Two antibiotic metabolites were isolated from cultures of Leucoagaricus carneifolia. Their structures were elucidated by spectroscopic methods. The first compound, 7-chloro-4,6-di-methoxy-1(3 H)-isobenzofurane (1) had to our knowledge not been described from natural sources whereas the second, basidalin (2), is a known metabolite of L. naucina (H. Iinuma et al., 1983). 1 exhibits antibiotic activities with minimal inhibitory concentrations of 20 μg/ml against Botrytis cinerea, the most sensitive microorganism

7-Chloro-4,6-dimethoxy-1(3H)-isobenzofurane and Basidalin: Antibiotic Secondary Metabolites from Leucoagaricus carneifolia Gillet (Basidiomycetes)