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The selection of plants resistant to photosynthetic herbicides is limited by the low rate of success in obtaining a photosynthetic callus. An attempt was made to induce diuron tolerance with chlorophyllous tissue strains of Bromus erectus: growth, chlorophyll contents and total fatty acids were studied during four months of photoheterotrophic growth in the presence or absence of 6 μM diuron. The presence of the herbicide, which reduced the fresh weight by 40%, provoked increases in chlorophyll levels by 2-or 3-fold, and in galactolipids (especially DGDG) which were enriched in linolenic acid content. These results, closely related to already described characteristics of herbicide-resistant material, suggest that callus of Bromus erectus could constitute an interesting photosynthetic in vitro material.

Long-Term Effect of Diuron on Chlorophyllous Callus of Bromus erectus: Lipid Composition