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м  Cu 2+ , 1 m м  Zn 2+ , and various re­ agents that react with sulfhydryl groups. Among the substrates tested for F3XT , kaempferol was the best. The K m  values for kaempferol and UDP-xylose were determined to be 0.83 jim and 25  μ M, respectively. F3XT mediated the transfer of xylose exclusively to the 3-hydroxyl group of kaempferol. Isorhamnetin, quercetin and fisetin also can function as xylosyl acceptor though less efficiently, but neither the 7-O-glucosides nor the 3-O-glucosides of kaempferol and quercetin were able to accept D-xylose. Dihydroflavonols were not xylosylated.

UDP- D -Xylose: Flavonol 3-O-Xylosyltransferase from Young Leaves of Euonymus alatus f. ciliato-dentatus