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Oligonucleotide-directed mutagenesis techniques were used to delete the psbF gene, encoding the β subunit of the cytochrom e b 559  protein of the photosystem II complex in the cyano­ bacterium, Synechocystis 6803. Cyt b 559  is an integral com ponent of PS II complex. However, its precise functional role in PS II remains to be determined. Previously, we created a mutant in which the psbF gene as well as three of its neighbouring genes, psbE , psbL and p sb i were simultaneously deleted from the chrom osom e of Synechocystis 6803 (Pakrasi, Williams and Arntzen, EMBO J. 7, 325 -332 , 1988). This mutant had no PS II activity. However, the role of any one of the four individual gene products could not be determined by studying this mutant. The newly generated mutant, T 256, had only one gene, p sbF , deleted from the genome. This mutant was also impaired in its PS II activities. In addition, it had barely detectable levels of two other protein com ponents, D1 (herbicide binding protein) and D2, of the reaction center of PS II, in its thylakoid membranes. In contrast, two other proteins of PS II, CP47 and CP43 were present in appreciable amounts. Fluorescence spectra (77 K) of the mutant showed the absence of a peak at 695 nm that was previously believed to originate from CP47. In addition, phycobilisomes, the light-harvesting antenna system of PS II, were found to be assembled normally in this mutant. We conclude that the presence of the β subunit of Cyt b 559  in the thylakoid membranes is critically important for the assembly of PS II reaction center.

Targeted Deletion Mutagenesis of the β Subunit of Cytochrome b 559 Protein Destabilizes the Reaction Center of Photosystem II