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Incubation of synthetic 12-hydroxy-(3 Z)-dodecenal (3 ZHD) with tea chloroplasts revealed that 12-hydroxy-(2 E)-dodecenal (2 EHD) was the product of isomerization reaction of 3 ZHD during the incubation. The isomerization was caused also by incubation of 3 ZHD with the heat-inactivated chloroplasts. The isomerization activity was extracted with hot ethanol. These results indicate that the isomerization was caused by heat-stable factor(s) but not by enzyme(s) in tea chloroplasts.

zeitschrift für naturforschung c

Non-Enzymatic Isomerization of 12-Hydroxy-(3Z)-dodecenal to the (2JE)-Isomer after Enzymatic Cleavage of 13-HydroperoxylinoIeyl Alcohol in Tea Chloroplasts