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A new polyclonal antiserum against spinach CF 1  subunit delta was produced in rabbits. It decorates only one band at 21 kDa in Western immunoblots of thylakoid proteins and does not react in ELISA with δ-free four subunit CF 1  (-δ); therefore it is regarded monospecific. The polypeptide used as immunogen had been purified by HPLC. Earlier antisera against CF 1  δ crossreact with CF 1  subunit β.

zeitschrift für naturforschung c

Localization and Orientation of Subunit Delta of Spinach Chloroplast ATP-Synthase within the CF 0 CF 1  Complex. 1. Distinction of Shielded and Exposed Surfaces of Delta on the Thylakoid Membrane