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The polynucleotides poly(U), poly(C), poly(A) and poly(G) have been y-irradiated in N 2 O- and N 2 O/O 2  (4:1)-saturated aqueous solutions. Hydroxyl radicals from the radiolysis of water react with the polynucleotides thereby producing among other lesions strand breaks. Strand breakage is connected with the formation of phosphomonoester end groups. Such end groups have been determined by measuring inorganic phosphate after a three hour incubation at 37 °C with acid or alkaline phosphatase.

The Formation of Phosphate End Groups in the Radiolysis of Polynucleotides in Aqueous Solution