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The application of the thin-agar-layer coated filter culture technique (Z. Naturforsch. 42c, 1082 (1987)) has been extended to the detection of ester hydrolysis and 2-hydroxyacid oxidation by microbial colonies. The former was performed by spraying with bromocresol purple and the latter with a salicylhydrazide reagent. Under the optimized conditions, the hydrolysis activity of more than 20 µmol/h·g-wet cells and the oxidation activity of more than 40 µmol/h·g-wet cells were usually detected directly on the filter-plate cultures of bacteria, yeasts and molds.

zeitschrift für naturforschung c

Entwicklung neuer Plattentests zum Nachweis mikrobieller Hydrolysen von Estern und Oxidationen von 2-Hydroxycarbonsäuren / Development of New Plate Tests for the Detection of Microbial Hydrolysis of Esters and Oxidations of 2-Hydroxycarboxylic Acids