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The grass Trisetum flavescens (golden oat grass, Goldhafer) causes soft tissue calcification in cattle and in sheep. The calcinogenic principle of the plant is the active vitamin D steroid hormone 1,25-Dihydroxyvitamin D 3 , the major physiological regulator of calcium homeostasis in higher animals. From comparison with synthetic vitamin D metabolites in different bioassays, it is concluded that T. flavescens contains the 25-glucoside of 1,25(OH) 2 D 3 . This compound, or rather the 1,25(OH) 2 D 3  liberated by ruminal fluid, is the calcinogenic factor of the grass.

A Vitamin D 3 Steroid Hormone in the Calcinogenic Grass Trisetum flavescens