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The incorporation of [1,2- 13 C 2 ]acetate into the bacterial phytotoxin pyoluteorin (1) as determined by  13 C NMR spectroscopy occurs in a pattern consistent with the biosynthesis of the molecule as a tetraketide in which proline, or an equivalent precursor derived from the TCA cycle, serves as the starter unit.

Biosynthesis of Pyoluteorin: A Mixed Polyketide-Tricarboxylic Acid Cycle Origin Demonstrated by [1,2- 13 C 2 ]Acetate Incorporation