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The basidiomycete Lentinellus cochleatus CBS 201.47 was cultivated on a defined synthetic liquid culture medium containing glucose (2%), asparagine (0.15%), malt extract (0.2%), and mineral salts. 12 weeks old cultures produced a “sweet” odour with an anise-or cinnamon-like note. 3 major volatiles could be identified from the steam distillates by their GC/MS-and  1 H NMR data. Predominant constituents were the acyclic sesquiterpene alcohols trans-nerolidol (41.4%) and fokienol (14.8%). 2,2-Dimethyl-6-formylchromene (6.1%) is described for the first time as a natural product. Besides these, 15 minor constituents could be identified or chemically charac­terized, respectively, the majority of them being sesquiterpene hydrocarbons and alcohols.

Volatiles from Liquid Cultures of Lentinellus cochleatus (Basidiomycotina)