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12-O-Tetradecanoylphorbol-13-acetate (TPA, 100 ng ml -1 ), a tumor promoting phorbol ester, is able to induce enhanced levels of the transformation-associated cellular antigen p53 in normal rat2 cells which had not been previously initiated by a carcinogen. p53 was estimated in ethanolfixed treated cells on microtiter plates with ELISA using the monoclonal antibody Pab 1620 [EMBO J. 7, 1485, (1984)]. Induction of p53 was confirmed by immunoblotting. This effect of TPA is an additional phenotypic characteristic of tumor cells which can be induced by TPA in untransformed rodent cells.

zeitschrift für naturforschung c

Transformation-Related Cellular Protein p53: Increased Level in Untransformed Rat Cells Following Treatment with the Tumorpromoter, Tetradecanoylphorbol-Acetate